Regulatory

Part:BBa_K4752004:Design

Designed by: Daorini, Youyan Liu, Yanlin Ren   Group: iGEM23_HNU-China   (2023-10-06)


GLR2.9 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 630
    Illegal EcoRI site found at 1257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 630
    Illegal EcoRI site found at 1257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 630
    Illegal EcoRI site found at 1257
    Illegal BglII site found at 1067
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 630
    Illegal EcoRI site found at 1257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 630
    Illegal EcoRI site found at 1257
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5


Design Notes

This part comes from Arabidopsis. GLR2.9 gene (gene lD:817458) was initially identified, and about 1500bp upstream of the gene was selected to cover the promoter region, and this part was prepared by PCR. To facilitate system assembly, we added appropriate homologous arms and restriction enzyme recognition sites.

Source

This part is originated from Arabdopsis. We prepare this part through PCR

References